Preparation and study of fragments of single rabbit nephrons.
نویسندگان
چکیده
BURG, M.,J. GRANTHAM, M. ABRAMOW, AND J. ORLOFF. Preparation and study of fmgments of single rabbit nephrans. Am. J, Physiol. 210(6): 1293-1298. rg66.-A method has been developed for the dissection and in vitro study of fragments of single rabbit nephrons. Fragments of different tubule segments 3-4 mm long were dissected from set tions of kidney immersed in oxygenated Ringer solution. Electrolyte and water composition of proximal tubule fragments were determined after incubation in appropriate solutions. It was found that isolated proximal tubules maintain large transcellular concentration gradients for Na, K, and Cl similar to kidney slices and mixed suspensions of kidney tubules. In order to measure transcellular transport, individual tubule segments were perfused and changes in the volume and composition of the effluent perfusion fluid measured. The viability of perfused proximal tubules was demonstrated by the presence of both active PAI-I transport and net Auid absorption.
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عنوان ژورنال:
- The American journal of physiology
دوره 210 6 شماره
صفحات -
تاریخ انتشار 1966